What is HPTLC?

[etcetera]

These are the articles I found and they are all full of science talk so I’m having trouble understanding, however, I’ll give it a shot, HPTLC is a refined form of TLC and is a preferable alternative to high-performance liquid chromatography (HPLC) and gas chromatography (GC). As far as I could understand it, it works by spreading out a sample on a plate then putting it inside the machine and letting it do its thing. For more info look up TLC or thin-layer chromatography.

Edit: Upon further investigation, I’ve found out that the exact procedure is that they shine a light on the sample and measure the frequencies of light that returns and this allows them to find out roughly what is inside the sample.

If you need more explaining on the topic of color: https://www.youtube.com/watch?v=oTDc5kmrEfs

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658041/

https://whatishplc.com/hplc-basics/principle-and-procedure-of-hptlc-chromatography/

https://www.emdmillipore.com/US/en/analytics-sample-preparation/learning-center-thin-layer-chromatography/hptlc/NGub.qB.fCoAAAFVPmJDx07N,nav?ReferrerURL=https%3A%2F%2Fwww.google.com%2F

https://en.wikipedia.org/wiki/High-performance_thin-layer_chromatography

• This reply was modified 1 year, 7 months ago by  Etcetera.
• This reply was modified 1 year, 7 months ago by  Etcetera.

[BlackoutKnight]

So for the first question the test is done by Piezoelectric tests machine which separates the material allowing them to see the difference between the samples. my answer to the second question is chemistry and physics. Well every thing ties back to physics electrons move at speeds that only let some colors through this is called chromatography.

[profmag]

Let’s try to get the science talk out of this and make it pretty simple. HPTLC is based on the following principles:

• separation by density
• different rates of absorption of different substances

If you wanted to know what was in the soil in your yard, one way you could figure out the components would be to dump a lump of dirt into water, stir it violently, and let it settle in to layers. These layers would be mainly based on density with the most dense material settling first and the least dense settling last.

HPTLC does the same sort of thing to sort out components. You dissolve the sample in a solvent (the mobile phase), and then you carefully pour it down a tray of silicone gel. As some of it gets absorbed quickly and some less quickly, it will separate into bands of components. These bands can then be compared with reference samples to see if the mixture matches a known substance.

[profmag]

I definitely messed you guys up earlier today with my definition of chromatography. I was totally off. What I was describing to you was actually spectrometry. It’s a really cool way of analyzing materials, but it has nothing to do with chromatography which is just separating materials into component parts and capturing that separation for analysis.